Limulus assay for bacterial endotoxin in synovial fluid.
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چکیده
منابع مشابه
Limulus assay for bacterial endotoxin in synovial fluid.
The limulus assay for the detection of bacterial endotoxin has been applied to the study of synovial fluid. Three of 5 patients (60%) with culture-positive gonococcal arthritis had positive SF limulus assay results; as did 2 of 11 (18%) with presumptive evidence of gonococcal arthritis, 3 of 6 (50%) with nongonococcal infectious arthritis, and none of 47 patients with noninfectious arthritis. E...
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A new reagent for the chromogenic Limulus amebocyte lysate (LAL) assay is described. LAL was formulated for optimal performance in either an endpoint procedure or a kinetic procedure with the chromogenic substrate, buffer, and LAL components colyophilized as a single reagent. The kinetic chromogenic method required an incubating microplate reader coupled to a computer for collection and analysi...
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A Limulus amoebocyte lysate microtechnique performed in petrolatum wells on a microscope slide is described. Injection of a dye solution in ethanol directly into the wells leads to an unambiguous interpretation of the results. Twelve samples can be tested on a single slide, and compact storing of the samples is possible.
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The Limulus amoebocyte lysate endotoxin assay was evaluated as a method for rapid diagnosis of acute bacterial meningitis in a series of 305 patients. The results of Limulus assays on cerebrospinal fluid (CSF) samples from these patients were compared with the results for each patient of routine bacterial cultures and Gram stains. Positive Limulus tests were obtained on initial CSF specimens fr...
متن کاملSynovial inflammatory response to bacterial endotoxin.
The ability of bacterial endotoxins to produce local inflammation has been recognized as one of the many biologic activities of these intriguing products of the gram-negative bacterial cell.1" This property of endotoxin, however, has not been studied extensively with the highly purified materials now available. Preliminary observations are reported here on the inflammatory response elicited by ...
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ژورنال
عنوان ژورنال: Annals of the Rheumatic Diseases
سال: 1983
ISSN: 0003-4967
DOI: 10.1136/ard.42.5.571